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Efficient callus induction and
plant regeneration from anther of Chinese narcissus (Narcissus tazetta
L. var. chinensis Roem).
Chen
L, Zhu
X, Gu
L, Wu
J.
Department of Biology, School of Life Science, Xiamen
University, Xiamen 361005, China. linjiaochen@163.com
Callus
culture has, to date, been reported only in a few species of Narcissus.
We used anthers of Chinese narcissus (Narcissus tazetta L. var.
chinensis Roem) as explants for callus induction and plant regeneration.
A high percentage of anthers at the early- to mid-uninucleate microspore
stage were responsive on the basal MS medium supplemented with 0.5-1 mg
l(-1) 2,4-dichlorophenoxyacetic acid and 0.5-2 mg l(-1) 6-benzyladenine
under dark conditions. Calli were initiated from anther connective
tissue or anther wall tissue, and no division of microspores occurred
during callus formation, as determined by histological observation.
Using 20 random amplified polymorphic DNA primers, we verified the
genetic integrity of the anther-derived plants of Chinese narcissus with
respect to the donor plants. These results suggest that anther culture
in vitro can provide an efficient new micropropagation technique for
Chinese narcissus as well as a new strategy for in vitro mass
propagation of other daffodils.
PMID: 15843934 [PubMed - indexed
for MEDLINE]
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